How to identify curcumin turmeric extract?

1. Conventional method
The ethanol solution of color reaction A sample should be pure yellow and have light green fluorescence; if this ethanol extract is added with concentrated sulfuric acid, it will produce deep purple red. Color Reaction B Treat an aqueous or dilute ethanol solution of the sample with hydrochloric acid until it begins to turn slightly orange.

Divide this mixture into two parts, and add a small amount of boric acid powder or crystals to one part, which turns reddish significantly compared to the part without boric acid. Several pieces of filter paper can also be dipped in the ethanol solution of the pigment, dried at 100°C, and then wetted with a dilute solution of boric acid (with a few drops of hydrochloric acid added). It should be cherry red when dry..

2. Chromatography

Take 5 ml of the test solution (0.01 g sample dissolved in 1 mL of 95% ethanol) and drop it on thin layer chromatography (microcrystalline cellulose, 0.1 mm), and put it in a container containing 3-methyl-1-butanol/ethanol/water/ In the development tank of ammonia (4:4:2:1) mixture, the solvent front is raised by 10-15 cm. Observation under ultraviolet light after one day and night: there are three yellow spots with Rf between 0.2 and 0.4; the Rf of other spots is 0.6 to 0.8; all spots show yellow fluorescence under ultraviolet light. 6.95% ethanol solution has a maximum absorption peak at 425 nm.

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2022-6-16

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